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  • This invention is a broadly applicable biosensor platform for detecting protein-protein interactions in eukaryotic and mammalian cells in situ or in vitro. This system uses small beta-lactamase fragments to perform assays on molecules, irrespective of their localization within the cell. In addition, detection with a fluorescent cell-permeable substrate provides a low-background signal. The technology can be used for diagnostics as well as other applications.

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