Heme oxygenase transgenic reporter mouse en
HO-1-luc 15 as a transgene in mice. A 15kb HO-1 promoter (provided by Y. Alam) and the entire luciferase coding sequence from pGL3-Basic vector (Promega) were cloned into the plasmid pBluescript KS(+) (Strategene). This construct was introduced into mouse embryos (strain FVB) and the animals were bred to homozygosity. These mice resond, with light emission that can be detected externally, to the presence of heavy metals and metalloporphyins and to physiologic stresses such as hyperoxia. [ - ]
Links
| Subject | Predicate | Object/Value | Creator | Attribution | Timestamp | |
|---|---|---|---|---|---|---|
| 1 | /m/0h99qkm | /common/topic/description | HO-1-luc 15 as a transgene in mice. A 15kb HO-1 promoter (provided by Y. Alam) and the entire luciferase coding sequence from pGL3-Basic vector (Promega) were cloned into the plasmid pBluescript KS(+) (Strategene). This construct was introduced into mouse embryos (strain FVB) and the animals were bred to homozygosity. These mice resond, with light emission that can be detected externally, to the presence of heavy metals and metalloporphyins and to physiologic stresses such as hyperoxia. /lang/en | /user/wdsnow | none | |
| 2 | /m/0h99qkm | /type/object/type | /projects/project_focus | /user/tvd | none | |
| 3 | /m/0h99qkm | /projects/project_focus/projects | /m/0jshbgl Development of: Heme oxygenase transgenic reporter mouse (HO Luc Mouse) | /user/tvd | none | |
| 4 | /m/0h99qkm | /common/topic/topical_webpage | http://otlportal.stanford.edu/techfinder/technology/ID=22619 | none | /user/gardening_bot/attr/563 | |
| 5 | /m/0h99qkm | /law/invention/date_of_invention | 2000 | /user/scottvanduyne | none | |
| 6 | /m/0h99qkm | /base/visleg/labeled/subject | S00-048 /lang/en | /user/wdsnow | none | |
| 7 | /m/0h99qkm | /type/object/type | /base/visleg/topic | /user/wdsnow | none | |
| 8 | /m/0h99qkm | /type/object/type | /base/visleg/labeled | /user/wdsnow | none | |
| 9 | /m/0h99qkm | /law/invention/inventor | /m/0fp9zz9 Christopher H. Contag | /user/wdsnow | none | |
| 10 | /m/0h99qkm | /law/invention/inventor | /m/0h99frg David K. Stevenson | /user/wdsnow | none | |
| 11 | /m/0h99qkm | /law/invention/inventor | /m/0h99dr0 Pamela R. Contag | /user/wdsnow | none | |
| 12 | /m/0h99qkm | /type/object/type | /law/invention | /user/wdsnow | none | |
| 13 | /m/0h99qkm | /common/topic/webpage | /m/0h99qm8 cvt | /user/wdsnow | none | |
| 14 | /m/0h99qkm | /common/topic/image | /m/0gtml_d OTL.png | /user/wdsnow | none | |
| 15 | /m/0h99qkm | /common/topic/article | /m/0h99qld | /user/wdsnow | none | |
| 16 | /m/0h99qkm | /type/object/permission | /boot/all_permission | /user/wdsnow | none | |
| 17 | /m/0h99qkm | /type/object/type | /common/topic | /user/wdsnow | none | |
| 18 | /m/0h99qkm | /type/object/name | Heme oxygenase transgenic reporter mouse /lang/en | /user/wdsnow | none | |
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