This invention describes structures, and a method for the production, of a universal nucleic acid linker, comprised of a functional group linked to a polynucleotide through an activating leaving group and a tether. Such functional groups can be fluorescence quenchers, fluorochromes, prodrugs, drugs, among others, and can provide for detection of chemical changes following their release from the modified polynucleotide. These linkers can be used to modify polynucleotide probes in ligation reactions, as well as transfer reactions. The probes are useful in a variety of reactions triggered by specific hybridization to a target nucleic acid, and can be used with in vitro and in vivo applications to detect and quantify nucleic acid target sequences. The linkers also promote fast reaction and amplification of signals.This technology works together with Prof. Kool's other invention Quenched DNA probes that "light up" on sensing genetic sequences' (S01-203).A co-exclusive license is available for all molecular in-vivo, fluorescent applications for internal research use and commercial reagents, diagnostics and diagnostic services. [ - ]

This invention describes structures, and a method for the production, of a universal nucleic... [ + ]