Stanford researchers have developed a method to quantify a cell phenotypic response over a continuous range of ectopic gene expression levels. The researchers generated a library of synthetic promoters capable of a 40-fold expression range in mammalian cells and developed a retroviral vector to faithfully deploy the library, gene of interest, and fluorescent reporter. This invention is fast, efficient, and highly compatible with existing expression systems since the promoters have been generated from constitutive promoters already commonly used in practice. This invention will allow experiments that generate and evaluate a range of gene expression levels. These types of experiments have been limited in the past since currently available tools and methods are unavailable, tedious, or inadequate. [ - ]

Stanford researchers have developed a method to quantify a cell phenotypic response over a... [ + ]